Journal: bioRxiv

Article Title: Safety and efficacy of a new vaginal gel, Feminilove, for the treatment of symptoms associated with vaginal dryness and vulvovaginal atrophy in women: an in vitro and in vivo study

doi: 10.1101/2021.08.17.455703

Figure Lengend Snippet: Lack of membrane damaging effect of Feminilove vaginal gel on reconstituted human vaginal epithelia. in vitro 3-D tissue cultures of human vaginal epithelial cells grown in polycarbonate membrane filters were exposed to Feminilove vaginal gel in culture medium (left column) or topically via gel (right column) and toxicity was evaluated by histological assessment of H&E-stained tissue sections. Cultures were exposed to 0, 0.01, 0.1, or 1% Feminilove vaginal gel for 24 hours.

Article Snippet: We purchased 3 human epithelial cells lines (vaginal cell line VK2/E6E7, endocervical End/E6E7 and ectocervical Ect/E6E7) from ATCC for this study.

Techniques: Membrane, In Vitro, Staining

Journal: bioRxiv

Article Title: Safety and efficacy of a new vaginal gel, Feminilove, for the treatment of symptoms associated with vaginal dryness and vulvovaginal atrophy in women: an in vitro and in vivo study

doi: 10.1101/2021.08.17.455703

Figure Lengend Snippet: Change in bacterial growth with exposure to commercial vaginal moisture products. (A) Final change in growth as measure by OD600, for laboratory strain of E.coli. All products exhibit similar inhibitory properties of E. coli. (B) Final change in growth of L. crispatus, as measured in CFU (simple co-culture without normal human vaginal epithelial cell, Line Cell Technology, FC-0083). Results are presented as the means and bars represent standard deviation from 3 independent experiments in which each condition was tested in triplicate.

Article Snippet: We purchased 3 human epithelial cells lines (vaginal cell line VK2/E6E7, endocervical End/E6E7 and ectocervical Ect/E6E7) from ATCC for this study.

Techniques: Co-Culture Assay, Standard Deviation

Journal: NPJ Biofilms and Microbiomes

Article Title: Extracellular vesicles from vaginal Gardnerella vaginalis and Mobiluncus mulieris contain distinct proteomic cargo and induce inflammatory pathways

doi: 10.1038/s41522-024-00502-y

Figure Lengend Snippet: bEV preparations from ( a ) NYC culture medium (control), ( b ) L. crispatus , ( c ) G. vaginalis , and ( d ) M. mulieris were labeled with rhodamine B isothiocyanate and observed in the cytoplasm of ectocervical (Ect1), endocervical (End1), and vaginal epithelial (VK2) cells after 1, 4, and 24 h of incubation. All scale bars are 20 μm.

Article Snippet: Ectocervical (Ect/E6E7, ATCC CRL-2614), endocervical (End1/E6E7, ATCC CRL-2615), and vaginal (VK2/E6E7, ATCC CRL-2616) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in keratinocyte-serum-free media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 μg/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Control, Labeling, Incubation

Journal: NPJ Biofilms and Microbiomes

Article Title: Extracellular vesicles from vaginal Gardnerella vaginalis and Mobiluncus mulieris contain distinct proteomic cargo and induce inflammatory pathways

doi: 10.1038/s41522-024-00502-y

Figure Lengend Snippet: IL-8 expression measured by ELISA increases with increasing doses of bEVs from G. vaginalis and M. mulieris , but not L. crispatus and NYC culture medium (control), relative to nontreated ( a ) ectocervical, ( b ) endocervical, and ( c ) vaginal epithelial cells. d Exposure to bacterial EVs does not induce significant changes in lactate dehydrogenase release. 29-plex Luminex array identified a multi-cytokine response to bEVs from G. vaginalis and M. mulieris , but not L. crispatus , expressed as fold-changes relative to NYC culture medium control-treated ( e ) ectocervical, ( f ) endocervical, and ( g ) vaginal epithelial cells ( n = 3 per treatment). Error bars indicate standard deviation and asterisks indicate adjusted p -values < 0.05 (*), < 0.01 (**), < 0.001 (***), and <0 .0001 (****) via one-way ANOVA with Tukey’s correction for multiple comparisons.

Article Snippet: Ectocervical (Ect/E6E7, ATCC CRL-2614), endocervical (End1/E6E7, ATCC CRL-2615), and vaginal (VK2/E6E7, ATCC CRL-2616) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in keratinocyte-serum-free media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 μg/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Luminex, Standard Deviation

Journal: Cell reports

Article Title: A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis

doi: 10.1016/j.celrep.2019.02.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The ectocervical Ect1/E6E7 cells (ECT, #CRL-2614) and SiHa cervical cancer cell line (SiHa, #HTB-35) were from ATCC (Manassas, VA).

Techniques: Virus, Microarray, Recombinant, Reverse Transcription, SYBR Green Assay, Plasmid Preparation, Staining, Blocking Assay